y 27632 (MedChemExpress)

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MedChemExpress y 27632
Y 27632, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y+27632/Y-27632/pmc13695517-156-10-12
Average 99 stars, based on 730 article reviews

y 27632 - by Bioz Stars, 2026-11

99/100 stars

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Cell Culture:

Article Title: Power-law scaling of mitotic spindles with genome sizes across eukaryotes is driven by chromosome crowding.
Article Snippet: Dissociated single cells and clumps were plated in Matrigel domes on 35-mm uncoated dishes with 0.17-mm glass thickness (MatTek Corporation), covered with 500 μl of the IntestiCult medium and cultured for 24–96 h prior to fixation. .. Samples fixed 24 h post-isolation were cultured with the addition of 10-μM Y-27632 (ROCK inhibitor, HY-10583, MedChemExpress) and 1 h before the fixation the inhibitor was washed out. ..

Transfection:

Article Title: Sequential TPRX1 and HERVL activation delineates a progression toward the totipotent-like state.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TPRX1 antibody Sigma Cat# HPA044922; RRID: AB_10962509 Monoclonal Mouse anti-Human ZSCAN4 Antibody Lsbio Cat# C175371; RRID: N/A Anti-KLF17 antibody produced in rabbit Sigma Cat# HPA024629; RRID: AB_1668927 OCT4 Monoclonal Antibody Thermo fisher Cat# MA5-14845; RRID: AB_10979606 Donkey Anti-Mouse IgG H&L (Alexa Fluor 647) Abcam Cat# ab150107; RRID: AB_2890037 Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) Abcam Cat# ab150075; RRID: AB_2752244 Deposited data bulk_RNA-seq This paper GSE309040; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE309040 ATAC-seq This paper GSE309041; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE309041 scRNA-seq This paper GSE309145; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE309145 bulk_RNA-seq of human embroyos Xiang et al.10 GSE268270; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE268270 bulk RNA-seq of human oocytes and preimplantation embryos Zou et al.12 HRA002463; GSA-Human; controlled access by application; https://ngdc.cncb.ac.cn/ gsa-human/browse/HRA002463 scRNA-seq of human embryos Petropoulos et al.20 E-MTAB-3929; BioStudies ArrayExpress; https://www.ebi.ac.uk/biostudies/ arrayexpress/studies/E-MTAB-3929 scRNA-seq of human embryos Yan et al.19 GSE36552; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE36552 ATAC-seq of human embryos Wu et al.21 GSE101571; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE101571 scRNA-seq of hTBLC Li et al.6 GSE224794; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE224794 scRNA-seq of e4CLs cells Mazid et al.7 CNP0001454; CNGBdb; https://db.cngb.org/ data_resources/project/CNP0001454/ Chemicals, peptides and recombinant proteins Matrigel Corning Cat# 354230 ReLeSR Stemcell Technologies Cat# 100–0483 EDTA Invitrogen Cat# 15575020 N2 Gibco Cat# 17502048 B27 Gibco Cat# 17504044 sodium pyruvate Gibco Cat# 11360070 non-essential amino acids Gibco Cat# 11140050 GlutaMAX Gibco Cat# 35050061 penicillin-streptomycin Gibco Cat# 15140122 DZNep MedChemExpress Cat# HY-12186 TSA MedChemExpress Cat# HY-15144 PD0325901 MedChemExpress Cat# HY-10254 IWR-1 MedChemExpress Cat# HY-12238 human LIF Peprotech Cat# 300–05 ACTIVIN A Peprotech Cat# 120-14E L-ascorbic acid Sigma Cat# 49752 (Continued on next page) 14 Cell Reports 45, 118163, November 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Geltrex Invitrogen Cat# A1413302 Accutase Gibco Cat# 00–4555-56 Y-27632 MedChemExpress Cat# HY-10583 Abexinostat MedChemExpress Cat# HY-10990 Acetate Invitrogen Cat# AM9740 LipofectamineTM Stem Transfection Reagent ThermoFisher Cat# STEM00001 RNAiMAX transfection reagent ThermoFisher Cat# 13778–100 DAPI Abcam Cat# ab104139 Total RNA Extraction Reagent Vazyme Cat# R401 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R323-01 PerfectStart Green qPCR SuperMix Transgen Cat# AQ601 Experimental models: Cell lines Human: H9 WiCell Cat# WA09 Human: CB0010 the National Stem Cell Resource Center (NSCRC), Institute of Zoology, Chinese Academy of Sciences Cat# CB0010 P15 Human: iPSC-3 the National Stem Cell Resource Center (NSCRC), Institute of Zoology, Chinese Academy of Sciences Cat# C07510031620230816019 Oligonucleotides RT-qPCR primers,see Table S1 gRNA sequences and ASO sequences, see Table S1 HERVL and MLT2A1 full length sequence, see Table S1 Recombinant DNA Plasmid:pSpCas9(BB)-2A-Puro (PX459) Addgene 62988 Plasmid: pLV hU6-sgRNA hUbC-dCas9KRAB-T2a-Puro Addgene 71236 Software and algorithms FlowJo v10.8.1 BD Biosciences N/A ImageJ National InstitutesofHealth https://imagej.nih.gov/ij GraphPadPrismversion 9.2 GraphPadSoftware https://www.graphpad.com/updates FastQC 0.11.9 Babraham Bioinformatics https://www.bioinformatics.babraham.ac.uk/ projects/fastqc MultiQC 1.13 Ewels et al.22 https://multiqc.info Trim Galore 0.6.6 Felix Krueger https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore STAR 2.7.4a Dobin et al.23 https://github.com/alexdobin/STAR featureCounts 2.0.1 Subread24 https://subread.sourceforge.net DESeq2 1.34.0 Bioconductor25 https://bioconductor.org/packages/DESeq2 clusterProfiler 4.2.2 Bioconductor26 https://bioconductor.org/packages/clusterProfiler Cell Ranger 3.7.1 10x Genomics https://www.10xgenomics.com/support/ software/cell-ranger Seurat 3.1.4 Satija Lab https://satijalab.org/seurat Monocle 2 2.26.0 Trapnell Lab27 https://cole-trapnell-lab.github.io/monocle-release Slingshot 2.18.0 Street et al.28 https://bioconductor.org/packages/slingshot Cutadapt 1.18 Marcel Martin29 https://cutadapt.readthedocs.io (Continued on next page) Cell Reports 45, 118163, November 24, 2026 15 .. Cell lines and ethical oversight The human pluripotent stem cell lines used in this study were H9 from WiCell and CB0010 and iPSC-3 from the National Stem Cell Resource Center (NSCRC), Institute of Zoology, Chinese Academy of Sciences.

RNA Extraction:

Real-time Polymerase Chain Reaction:

Stem Cell:

Quantitative RT-PCR:

Allele-specific Oligonucleotide:

Sequencing:

Recombinant:

Plasmid Preparation:

Software:

other:

Article Title: Deubiquitinase USP5 suppresses ferroptosis in lung adenocarcinoma by deubiquitinating and stabilizing GPX3
Article Snippet: Pre‐warmed organoid culture medium (IMV‐T18, IMMOCELL) supplemented with 10 μM Y‐27632 (HY‐10071, MedChemExpress, USA) was then added.

Inhibition:

Article Title: Identifying ROCK2 as an intervention target for bilirubin encephalopathy
Article Snippet: Four hours post-plating, the medium was replaced with Neurobasal-A medium (Gibco) supplemented with 1 × N2 (Invitrogen, USA), 1 × B27 (Invitrogen, USA), and 4 mM L-glutamine (Gibco, New Zealand). .. After 5 to 7 days in culture, neurons were exposed to bilirubin (25 μM) (Sigma-Aldrich, USA) for 24 h. To investigate the role of ROCK2 inhibition, Y-27632 (Med Chem Express, China) and Belumosudil (Med Chem Express, China) were dissolved in dimethyl sulfoxide (DMSO) (Solarbio, China) at a concentration of 50 mM and stored at −20 °C. ..

Concentration Assay:

Incubation:

Article Title: Improved Method for Obtaining Patient‐Derived Glioblastoma Organoid and Primary Cells: A Generic and Simplified Approach for Intracranial Tumor With Minimized Input
Article Snippet: .. The treated GBO pieces were incubated with culture medium containing 10 μM Y‐27632 (MedChemExpress, HY‐10071) for 1 h. The GBO pieces were resuspended in cryovials (LABSELECT, 22321029R) with culture medium containing 10% DMSO (Thermo Fisher Scientific, 85190) and 10 μM Y‐27632 at room temperature for 15 min. ..

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